RAD Microbes
Regenerative Agricultural Development LLC

A bioremediation strategy for the Wye, Usk and Lugg catchments.

Custom microbial consortia for the mitigation of phosphorus, nitrate and ammonia run-off across three Living Lab wetlands in the Wye Valley.

Prepared for
Simon CottonAquaprana Ltd
Prepared by
Barry BonnerRAD Microbes
Date
May 2026Confidential

Simon, thank you for the warmth and clarity of your brief.

Your February presentation made the case for nature-based treatment of agricultural run-off with exceptional rigour, and your three Wye Valley Living Labs sit at precisely the intersection of policy urgency, scientific opportunity, and community need that RAD Microbes was built to serve.

This document responds to your direct question: can RAD Microbes provide microbial products that are both process-efficient and cost-effective alternatives to the Next FOG Stop/FGX3 and Next-Sand components of your wetlands stack, given that US shipping costs have made the current specification prohibitive for UK farmers and funders.

The short answer is yes, and the longer answer is more interesting than a one-for-one product swap. What follows is not a trial pitch, it is a fully scoped bioremediation strategy. With Aquaprana acting as the proposed UK delivery partner, we are positioned to deliver site-specific microbial formulations into the Wye, Usk and Lugg catchments at a fraction of the shipped weight and volume of the current consumable stack, and at a frequency that drops sharply after initial inoculation. The result is a cost structure that DEFRA, the Wye and Usk Foundation, and the Environment Agency can sustainably fund at scale.

The proposition is straightforward. Phosphorus, nitrates, and ammonia in farm run-off are, fundamentally, a biological problem. They are best solved by introducing the right biology into the right places at the right time, and by designing the consortium around the specific pollutant load, hydrology, and substrate of each site rather than relying on a generic stock product.

A catchment under extraordinary nutrient pressure.

The Wye, Usk and Lugg are among the most ecologically significant river systems in the United Kingdom, and among the most acutely damaged by phosphorus loading from intensive poultry production and water utility discharge. The current High Court proceedings in London, which you referenced in your brief, are the legal expression of a crisis that has been building in the catchment for over a decade. The political and funding window for credible mitigation work has never been more open.

Against that backdrop, your wetlands design is the correct intervention. Filter trenches feeding a paired lagoon and reed bed, with controlled hydraulic retention and a floating baffle between zones, deliver the pollutant residence times needed to convert reactive nitrogen and bioavailable phosphorus into immobilised or atmosphere-bound forms. The architecture is sound. The cost barrier sits squarely in the consumable stack: the Next-Sand clinoptilolite media and the Next FGX3 protein surfactant complex, both shipped in bulk by weight and volume, both required season after season, both subject to a freight and re-supply economics that no farmer-funded or even DEFRA-funded scheme can sustain at catchment scale.

RAD Microbes resolves the consumable cost barrier through a fundamentally different model: a living, custom-formulated microbial consortium designed for your specific sites, fermented and stabilised at our partnered microbial genomics laboratory in the United States, and proposed for delivery into the UK with Aquaprana as the field partner, as a high-value, low-volume concentrate. The freight cost per hectare treated is structurally lower than the bulk consumable stack it replaces, and that gap widens season on season as the biology establishes and the re-inoculation interval extends.

What a properly designed microbial consortium does in a constructed wetland.

Constructed wetlands work because they create the spatial and temporal conditions for microbial communities to process pollutants biologically. The filter trench, lagoon, and reed bed each present distinct redox environments, substrate textures, and residence times, and each calls for a different microbial functional group.

The RAD Microbes bioremediation platform draws on thirty-seven years of microbial consortium development experience, with deep capability in the design, fermentation, and stabilisation of spore-forming bacterial communities. The platform's organisms are particularly well suited to wetland deployment for three reasons: they survive transport and variable hydration through dormant spore phases, they sit firmly within Biosafety Level 1 with established regulatory clearance for environmental release in most jurisdictions, and they perform a remarkably broad set of functions relevant to nutrient pollution mitigation.

The functional mechanisms relevant to your sites

Mechanism 01 Phosphate solubilisation and immobilisation
The consortium releases gluconic and citric acid that solubilises mineral-bound phosphorus, but in a constructed wetland the more valuable property is the reverse: the same biological infrastructure drives phosphorus uptake into biomass, biofilms, and stable organic forms, removing dissolved reactive phosphorus from the water column. This is the direct biological equivalent of the adsorption work currently being done by the Next-Sand clinoptilolite zeolite.
Mechanism 02 Ammonia oxidation pathway support
A properly designed wetland consortium establishes the rhizosphere conditions in which ammonia is biologically oxidised through to nitrate and then denitrified to atmospheric nitrogen gas. RAD Microbes will formulate the consortium to seed and stabilise these pathways at the reed root zone, where the oxygen gradient from aerenchyma transport meets the anaerobic sediment, which is precisely where denitrification happens at maximum efficiency.
Mechanism 03 Biosurfactant production
The consortium produces naturally occurring cyclic lipopeptide biosurfactants that lower surface tension, mobilise fats, oils, and greases, and enhance bioavailability of organic load for subsequent microbial processing. This is the functional role currently filled by the Next FGX3 protein surfactant complex, delivered by the living organisms themselves rather than as a continuously dosed external input.
Mechanism 04 Exopolysaccharide and biofilm formation
Consortium biofilms bind particulate matter and build stable aggregates within the filter trench Hessian sack medium, the lagoon bed, and the reed bed substrate. This improves particulate capture, extends contact time between pollutants and active biology, and progressively enhances treatment efficiency across successive seasons rather than degrading it.
Mechanism 05 Rhizosphere colonisation and plant performance
Indole-3-acetic acid production and root surface colonisation directly enhance Phragmites, Iris pseudacorus, Typha latifolia, and the other reed bed species in your planting plan, expanding the root mass that drives oxygen transport, nutrient uptake, and pollutant immobilisation. A healthier reed bed processes more nutrient load per square metre.
Mechanism 06 Pathogen suppression and system stability
The same antimicrobial compounds that protect the consortium against competition also suppress soilborne pathogens, reducing the risk of reed dieback or biofilm collapse during stress events such as storm surges, temperature extremes, or high pollutant load spikes from poultry shed wash-out.
Mechanism 07 Multi-season biological compounding
Unlike chemically dosed inputs, a living consortium establishes, reproduces, and adapts. Each season the biological infrastructure becomes more developed, more resilient, and more productive than the season before. The return on the initial inoculation compounds rather than resetting with each top-up.

How the consortium maps onto your existing wetlands design.

Your wetlands architecture is well-conceived and we do not propose changes to its physical structure. What we propose is a precise mapping of microbial functional groups onto the three treatment zones, with bespoke consortium formulations for each.

Treatment zone deployment

01
Filter trench
Consortium incorporated into the Hessian sack medium alongside pea shingle and a UK-sourced zeolite alternative. Initial colonisation of the trench substrate, first-stage biosurfactant action, particulate capture, and FOG mobilisation.
02
Lagoon
Lagoon-specific formulation supporting the aerobic processing layer above the substrate, optimised for the residence time achieved with vortex aeration. Targets dissolved phosphorus uptake and ammonia oxidation through the water column.
03
Reed bed
Rhizosphere-targeted formulation applied at planting and at seasonal re-inoculation, colonising the reed and companion species root zone where the aerobic and anaerobic gradient drives denitrification and the polishing stage of treatment.

For the Next-Sand clinoptilolite, we propose a hybrid approach. Clinoptilolite is a genuinely useful filtration and ion-exchange material, and we do not propose to fully replace it. We propose to source a UK or European zeolite equivalent (clinoptilolite is mined in Slovakia, Bulgaria, Turkey, and Greece, eliminating transatlantic shipping cost), and to reduce the volume required per hectare by allowing the biological consortium to take up the ammonia and phosphorus load that would otherwise rely entirely on physical adsorption. The two mechanisms are complementary, and a properly balanced biological-plus-mineral approach delivers better treatment per pound spent than either alone.

A three-phase delivery, sequenced to your programme.

RAD Microbes does not propose an off-the-shelf product. We propose a structured engagement that begins with site characterisation, moves into custom formulation and bench validation, and culminates in staged deployment across the three Wye Valley wetlands with monitoring built in from the first day.

I Phase

Site characterisation and pollutant profiling

Working with Aquaprana, RAD Microbes will compile baseline data on each of the three Wye Valley sites, including upstream pollutant load profile, dominant agricultural inputs, hydrology and seasonal flow variation, water chemistry, ambient temperature range, substrate composition, and any existing microbial community baseline. This is the foundation on which the formulation is designed and against which performance is later measured.

  • Baseline water quality sampling and laboratory analysis
  • Site visit and hydrological assessment in collaboration with Aquaprana
  • Engagement with Wye and Usk Foundation, DEFRA and Environment Agency contacts as appropriate
  • Documented site characterisation report per wetland
II Phase

Custom consortium formulation and bench validation

RAD Microbes will formulate three site-specific consortia under its bioremediation programme, one optimised for each treatment zone (trench, lagoon, reed bed). Each formulation will be validated under simulated wetland conditions before field deployment, with target pollutant reduction benchmarks established for phosphorus, ammonia, nitrate, and FOG.

  • Three custom formulations developed and stabilised in shelf-stable form
  • Bench-scale validation against site water samples
  • Application rate, dosing schedule, and re-inoculation interval specified per zone
  • Full technical documentation pack supplied to Aquaprana
III Phase

Staged deployment and longitudinal monitoring

Deployment proceeds in sequence across the three wetlands, with the first site serving as the lead case and the second and third receiving formulation refinements based on early data. Monitoring runs continuously across all three sites with quarterly reporting and annual performance reviews, designed to generate the evidence base needed for catchment-wide scale-up and ongoing public-sector funding submissions.

  • Inoculation of filter trenches, lagoons and reed beds per site
  • Quarterly water quality monitoring at inflow, mid-system and discharge points
  • Annual performance reports suitable for DEFRA, Environment Agency and Wye and Usk Foundation submission
  • Re-inoculation and formulation adjustment as data dictates

Six strengths that shape our offer to the project.

The Wye Valley Living Labs are a serious piece of environmental work, with serious funders, in a catchment whose decline is now well documented. The bar for any partner brought into a programme of this profile is high. The points below describe the specific strengths RAD Microbes would bring to the bioremediation layer of the work.

Strength 01 Custom site-specific formulation
RAD Microbes does not supply an off-the-shelf product. Each consortium is designed around the pollutant load, hydrology, substrate, and seasonal profile of the specific site it is being deployed into. The Wye Valley Living Labs would receive three formulations, one per site, refined against the actual baseline water chemistry rather than against assumptions or sector averages.
Strength 02 Multi-functional consortium
The platform does not rely on a single organism doing a single job. Our consortia bring together complementary functional groups across phosphorus solubilisation and immobilisation, ammonia oxidation support, biosurfactant production, biofilm and aggregate formation, rhizosphere colonisation, and pathogen suppression. The breadth of mechanism is what allows one biological system to do work that would otherwise require several different inputs.
Strength 03 Living biology, not enzymes or dead-cell preparations
What we supply is alive. The organisms establish, reproduce, and adapt to the conditions of the site they are introduced into. This is fundamentally different from enzyme-only products, dead-cell preparations, or chemically-dosed inputs, all of which work only for as long as they are continuously supplied. A living consortium develops over successive seasons rather than degrading.
Strength 04 Provenance from Deepwater Horizon
The microbial platform underpinning RAD Microbes traces its provenance to environmental remediation work conducted in response to the Deepwater Horizon incident, one of the largest applied bioremediation programmes ever undertaken. The biology was developed under real-world, large-scale, regulator-monitored conditions, not solely in laboratory settings. The same principles transfer directly to the nutrient pollution profile of a UK agricultural catchment.
Strength 05 Genomics-backed strain selection
Our fermentation, strain selection, and stabilisation work is conducted in partnership with a leading microbial genomics laboratory in the United States. This gives the platform direct access to genome-level strain characterisation, consortium design tooling, and aseptic production capacity at research-grade quality. Strain selection is evidence-led, not catalogue-led.
Strength 06 Architecture-specific deployment
The three zones of your wetland design, filter trench, lagoon, and reed bed, present distinct redox conditions, substrates, and residence times. Rather than apply a single product across all three, RAD Microbes would formulate three zone-specific consortia, each optimised for the biological work that zone is doing. The deployment maps onto the architecture you have already designed, without changes to the physical infrastructure.
In summary

A living, multi-functional, site-specific biological asset.

The strengths above are not separate claims, they are aspects of the same approach. A consortium that is custom-built for the site, biologically diverse rather than single-function, alive rather than chemically dosed, traceable to field-scale precedent, genomically characterised, and mapped onto the specific zones of the wetland design.

This is what RAD Microbes would bring to the bioremediation layer of the Wye Valley Living Labs programme, should the project proceed.

Thirty-seven years of applied microbial consortium development.

RAD Microbes is the trading name of Regenerative Agricultural Development LLC. The platform behind it draws on thirty-seven years of cumulative experience in microbial consortium development, built specifically around field-scale applications where the biology must perform under variable, uncontrolled, real-world conditions rather than under controlled laboratory ones.

That experience covers the full operational stack required for serious wetland bioremediation work: strain isolation and selection from natural environments, fermentation and aseptic production under pharmaceutical-grade protocols, proprietary stabilisation technology that preserves consortium viability through shipping and field deployment, Biosafety Level 1 verification, and field-scale application across agricultural soil restoration, rhizosphere enhancement, and aqueous remediation contexts.

RAD Microbes has developed consortia for plant rhizosphere applications with documented performance in nitrogen fixation, phosphorus and potassium solubilisation, biosurfactant production, soil aggregate formation, and pathogen suppression. These capabilities translate directly into the wetland context, applied at the reed bed root zone, the filter trench medium, and the lagoon substrate.

The UK delivery arrangement proposed with Aquaprana would provide the regulated channel for delivery into the Wye Valley and, looking forward, into wider UK catchment work, golf course greens trials, and the sports sector applications you flagged in your brief.

How we see the conversation developing from here.

We understand the Wye Valley Living Labs are at the funding and award stage, not the construction stage, and that any commitments on either side are contingent on the funding posture confirmed by the Wye and Usk Foundation, DEFRA, or the Environment Agency. This document is offered as a positioning statement on what RAD Microbes can bring to the project, designed to support your wider conversation with the funding bodies and your advisory team.

A working session

A working session between your group and ours, at a time that suits, to talk through the science, the formulation approach, the site-mapping logic, and the questions your team and your funding contacts will reasonably want answered. The objective of that session is alignment on whether the RAD Microbes approach fits the shape of the project you are building, before any commercial conversation begins.

If the funding is secured

If the Living Labs programme is awarded and the funding posture confirmed, RAD Microbes is positioned to move directly into site characterisation and bespoke formulation work for the lead Wye Valley site, with the second and third sites following on a rolling basis. The methodology set out in Section 04 of this document describes that path in detail.

Commercial structure

We have intentionally not addressed commercial terms in this document. The commercial structure is best developed in direct conversation between Aquaprana and RAD Microbes once scope is confirmed and the funding posture from the public-sector partners is clear. Our objective is a structure that works for the farmers, works for the funding bodies, and supports a sustainable long-term working relationship.

We look forward to your team's response and to building this programme alongside you.
Barry Bonner
Founder  ·  Regenerative Agricultural Development LLC
RAD Microbes  ·  Regenerative Agricultural Development LLC
Denton, Texas, USA
Proposed UK delivery partner: Aquaprana Ltd
RAD Microbes
Regenerative Agricultural Development
May 2026
Confidential
This document is strictly confidential and the sole property of Regenerative Agricultural Development LLC.

Confidentiality. This document and the information, data, methods, formulations, scientific claims, processes, and proposals contained within it are strictly confidential and are provided to the named recipient on the express condition that they will not be disclosed, copied, reproduced, distributed, summarised, paraphrased, transmitted, or otherwise shared, in whole or in part, with any third party, including but not limited to consultants, competitors, advisors, contractors, agents, public bodies, funding bodies, or affiliated entities, without the prior written consent of Regenerative Agricultural Development LLC.

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